Device

Part:BBa_K176091

Designed by: Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-08-10)


Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = (Cell Density * Input PoPS) + Input AHL

High Input PoPS -> Low Steady State Cell Density

High Input AHL -> Low Steady State Cell Density

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2280
    Illegal BsaI.rc site found at 1791


[edit]
Categories
Parameters
n/aCell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)